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文摘
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建立了花锚药材及其制剂中两种抗肝炎有效成分花锚甙和去甲氧基花锚甙的反相高效液相色谱测定方法.采用甲醇回流提取进行样品处理,在乙腈-磷酸水溶液为流动相作梯度洗脱、ODS柱、检测波长为254 nm条件下,花锚甙和去甲氧基花锚甙均可达到基线分离.两种成分在0.68～3.40 g/L,0.36～1.8 g/L时,其峰面积与浓度成良好的线性关系,加标回收率为95%～105%.该法适用于花锚药材及其制剂的质量分析检验. |
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其他语种文摘
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A simple and accurate method for the determination of haleniaside and demethoxy-haleniaside in Halenia ellipitica D. Don. materials and patent medicines with high performance liquid chromatography (HPLC) was developed. The two components were extracted from pow-dered samples with methanol. The resultant extract was separated within 45 min on a VP-ODS C_(18) column (4.6 mm i.d. *250 mm, 5μm) under gradient elution with a mixture of acetoni-trile-phosphate buffer at a flow rate of 1.0 mL/min. The detection wavelength was 254 nm and the injection volume was 20μL. Under gradient elution program the volume fractions of aceto-nitrile in mobile phase were as follows; 0-5 min, 12%; 5-15 min, 12% -15%; 15-40 min, 15% -35%. Both haleniaside and dehydrohaleniaside have good linearity in the ranges of 0.68 - 3.40 g/L and 0.36 - 1.8 g/L with correlation coefficients of 0.999 8 and 0.999 0 respectively. This method has been successfully applied to the analysis of Halenia ellipitica D. Don materi-als and related patent medicines. |
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来源
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色谱
,2004,22(1):38-40 【核心库】
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关键词
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高效液相色谱
;
花锚甙
;
去甲氧基花锚甙
;
花锚
;
乙肝健片
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地址
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1.
中国科学院西北高原生物研究所, 青海, 西宁, 810001
2.
青海普兰特藏药研究所, 青海, 西宁, 810007
3.
中国科学院西北高原生物研究所, 青海, 西宁, 810007
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-8713 |
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学科
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化学 |
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基金
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国家中西部专项基金
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文献收藏号
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CSCD:1506532
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