SSR水平上甘、青两省春小麦品种间的遗传多样性现状及演变趋势
Detection on evolvement and present situation of genetic diversity of spring wheat cultivars planted in Gansu and Qinghai Provinces by SSR markers

沈裕琥窦全文王海庆黄相国张怀刚

文摘	利用22对SSR引物的扩增结果计算品种间的Jaccard相似系数，在此基础上用UPGMA方法进行了聚类分析，检测了43个春小麦品种间在DNA水平上的遗传变异。22对引物共扩增出102条多态性带，平均每对引物可扩增出4.64条多态性带，具有较好的多态性。SSR水平上43个品种间遗传距离变异范围为0.2222～0.8393，平均遗传距离GD％=0.6054。43个品种聚为两大类，除佛手麦自成一类外，其余42个品种聚为第二大类。聚类结果真实地反映了品种间基因型差异。历史上地方品种间SSR水平上的遗传变异最大，育成品种遗传多样性水平总体上呈下降趋势，且低于地方品种和引进品种。1BL／1RS易位片段特异性引物Rye检测结果显示，共有7个品种含朋1RS片段，结果需进一步证实和深入研究。
其他语种文摘	102 polymorphic fragments were obtained in 43 accessions using 22 pairs of SSR primers. The av-erage number of polymorphic fragments at each SSR locus is 4. 64. There exists extensive variation among 43 accessions in SSRs. The range of genetic distance by SSRs is 0. 222 2~0. 839 3,and mean genetic dis-tance is 0. 605 5. Cluster analysis shows that 43 cultivars could be classified into 2 clusters at the level of GS 0. 26,and the other 42 cultivars are clustered together except Foshoumai. Clustering results reflect genotypic difference truly. The analysis of development of genetic diversity indicates that there exists ex-tensive variation among landraces, and genetic diversity among bred cultivars, which has the trend to de-crease in general,is slightly less than that of landraces and introduced cultivars. The result demonstrates that 7 cultivars contain IRS fragment using peculiar primer of 1BL/1RS translocation,which remains to be confirmed and probed further.
来源	西北植物学报 ,2003,23(10):1755-1761 【核心库】
关键词	春小麦 ; 品种 ; 遗传多样性 ; SSR
地址	中国科学院西北高原生物研究所, 西宁, 810001
语种	中文
文献类型	研究性论文
ISSN	1000-4025
学科	植物学
基金	瑞典国际科学基金（IFS）资助 ; 中国科学院西北高原生物研究所知识创新工程重点领域项目 ; 中共中央组织部人才培养基金 ; 中国科学院人才培养计划项目
文献收藏号	CSCD:1253737