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MC3T3-E1成骨细胞在微格式化表面的黏附
MC3T3-E_1 Osteoblasts Adhesion to Micropatterned Surfaces
查看参考文献12篇
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文摘
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细胞在材料表面的黏附对细胞的增殖和分化起重要作用。格式化表面提供了对细胞在基底的空间分布和黏附进行控制的方法。本文利用微制作形成的格式模板，分别以微接触转印法和微流道法形成格式化表面，使MC3T3-E1成骨细胞以一定的格式黏附于表面上。在微接触转印法形成的含二氯二甲基硅烷（DMS）的疏水区域和不含DMS的亲水区域相间隔的表面。细胞优先在亲水区域黏附。在微流道法形成的胶原和白蛋白格式化表面，细胞优先黏附于含胶原区域。结果还表明微格式化表面可以用于研究表面的物理化学性质对细胞的黏附等功能的影响。 |
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其他语种文摘
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Cell adhesion to material surface plays an important role in regulating cell function such as proliferation and differentiation. Surface patterning provides a useful method to control cell spatial distribution and adhesion to substance. Here microcontact printing and microfluidic channels were introduced to pattern MC3T3 E1 osteoblasts on silicon substance. Dichlordimethylsilane (DMS) was used in microcontact printing to generate the alternating domains of DMS and non-DMS, and cells preferentially adhered to the non-DMS and hydrophilic region. On the patterned surfaces generated from collagen and albumin solutions with microfluidic channels, cells preferentially localized in the collagen-coated region. The results also showed that micropatterning could be a useful method to study the effect of surface chemistry on cell adhesion and other functions. |
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来源
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生物医学工程学杂志
,2002,19(3):370-373 【核心库】
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关键词
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细胞黏附
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微接触转印
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微流道
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地址
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中科院力学研究所, 国家微重力室, 北京, 100080
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1001-5515 |
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学科
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基础医学 |
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文献收藏号
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CSCD:1067634
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参考文献 共
12
共1页
|
|
1.
Buckley C D. Cell adhesion: more than just glue.
Mol Membr Biol,1998,15(4):167
|
CSCD被引
1
次
|
|
|
|
|
2.
Healy K E. Kinetics of bone cell organization and mineralization on materials with patterned surface chemistry.
Biomaterials,1996,17:195
|
CSCD被引
6
次
|
|
|
|
|
3.
Britland S. Micropatterned substratum adhesiveness: a model for morphogenetic cues controlling cell behavior.
Experimental Cell Research,1992,198:124
|
CSCD被引
1
次
|
|
|
|
|
4.
Xia Y. Soft lithography.
Angew. Chem. Int. Ed,1998,37:550
|
CSCD被引
49
次
|
|
|
|
|
5.
Kane R S. Patterning proteins and cells using soft lithography.
Biomaterials,1999,20:2363
|
CSCD被引
18
次
|
|
|
|
|
6.
Chen C S. Micropatterned surfaces for control of cell shape, posotion, and function.
Biotechnol Prog,1998,14:356
|
CSCD被引
14
次
|
|
|
|
|
7.
Folch A. Molding of deep polymethylsioloxane microstructures for microfluidics and biological applications.
J Bioech Eng,1999,121:28
|
CSCD被引
1
次
|
|
|
|
|
8.
靳刚. 生物分子吸附膜层的图像显示-光学椭偏显微成像技术应用之一.
测试技术学报,1998,12(3):166
|
CSCD被引
10
次
|
|
|
|
|
9.
Dewez J L. Coupled influence of substratum hydrophilicity and surfactant on epithelial cell adhesion.
J Biomed Mater Res,1996,30:373
|
CSCD被引
1
次
|
|
|
|
|
10.
Curtis A S G. The competitive effects of serum proteins on cell adhesion.
J Cell Sci,1984,71:17
|
CSCD被引
1
次
|
|
|
|
|
11.
Lom B. A versatiole technique for patterning biomolecules onto glass coverslipos.
J Neuroscience Methods,1993,50:385
|
CSCD被引
1
次
|
|
|
|
|
12.
Singhvi R. Engineering cell shape and function.
Science,1994,264:6
|
CSCD被引
1
次
|
|
|
|
|
|
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